Interaction Details


Details of experiments between Q13136 and P31947
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PPFIA1
Fragment: 1134-1143
Site: 14-3-3_pSer1139
Modification: (SEP)1139
Sequence: FRRAPSWRKK
Construct: FRRAP(pSer)WRKK (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.18
Holdup BI standard deviation: 0.58
Immobilized partner concentration (10-6M): 142
P value: 0.33
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: PPFIA1
Fragment: 525-534
Site: 14-3-3_pSer530
Modification: (SEP)530
Sequence: RPHLGSVPDF
Construct: RPHLG(pSer)VPDF (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.32
Holdup BI standard deviation: 0.56
Immobilized partner concentration (10-6M): 113
P value: 0.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: PPFIA1
Fragment: 525-534
Site: 14-3-3_pSer530
Modification: (SEP)530
Sequence: RPHLGSVPDF
Construct: RPHLG(pSer)VPDF (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.22
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 142
P value: 1.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: PPFIA1
Fragment: 663-672
Site: 14-3-3_pSer668
Modification: (SEP)668
Sequence: RVGSGSLDNL
Construct: biotin-ttds-RVGSG(pSer)LDNL (HPLC-purified synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Measured dissociation constant (10-6M): below threshold
Experiment method: competitive Fluorescence Polarization
Details of affinity fitting: Competitive binding equation in ProFit
Date of measurement: 2022-02-09
Number of measurements: 3
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103

Experiment series 5

Protein A

Protein: PPFIA1
Fragment: 663-672
Site: 14-3-3_pSer668
Modification: (SEP)668
Sequence: RVGSGSLDNL
Construct: RVGSG(pSer)LDNL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.13
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 113
P value: 5.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: PPFIA1
Fragment: 663-672
Site: 14-3-3_pSer668
Modification: (SEP)668
Sequence: RVGSGSLDNL
Construct: RVGSG(pSer)LDNL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.17
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 142
P value: 4.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: PPFIA1
Fragment: 758-767
Site: 14-3-3_pSer763
Modification: (SEP)763
Sequence: ALHTVSHEDI
Construct: ALHTV(pSer)HEDI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.28
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 113
P value: 2.48
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.54
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: PPFIA1
Fragment: 758-767
Site: 14-3-3_pSer763
Modification: (SEP)763
Sequence: ALHTVSHEDI
Construct: ALHTV(pSer)HEDI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.2
Holdup BI standard deviation: 0.53
Immobilized partner concentration (10-6M): 142
P value: 0.48
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: PPFIA1
Fragment: 801-810
Site: 14-3-3_pSer806
Modification: (SEP)806
Sequence: KGIKSSIGRL
Construct: KGIKS(pSer)IGRL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.08
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 113
P value: 1.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: PPFIA1
Fragment: 801-810
Site: 14-3-3_pSer806
Modification: (SEP)806
Sequence: KGIKSSIGRL
Construct: KGIKS(pSer)IGRL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.09
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 142
P value: 1.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: PPFIA1
Fragment: 1154-1163
Site: 14-3-3_pThr1159
Modification: (TPO)1159
Sequence: AGSAETLPAN
Construct: AGSAE(pThr)LPAN (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.42
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 142
P value: 3.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.71
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: PPFIA1
Fragment: 1154-1163
Site: 14-3-3_pThr1159
Modification: (TPO)1159
Sequence: AGSAETLPAN
Construct: AGSAE(pThr)LPAN (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.35
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 113
P value: 1.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 13

Protein A

Protein: PPFIA1
Fragment: 567-576
Site: 14-3-3_pThr572
Modification: (TPO)572
Sequence: PSKVQTLNEQ
Construct: PSKVQ(pThr)LNEQ (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.02
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 113
P value: 0.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: PPFIA1
Fragment: 567-576
Site: 14-3-3_pThr572
Modification: (TPO)572
Sequence: PSKVQTLNEQ
Construct: PSKVQ(pThr)LNEQ (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 142
P value: 1.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 15

Protein A

Protein: PPFIA1
Fragment: 1134-1143
Site: 14-3-3_pSer1139
Modification: (SEP)1139
Sequence: FRRAPSWRKK
Construct: FRRAP(pSer)WRKK (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.06
Holdup BI standard deviation: 0.45
Immobilized partner concentration (10-6M): 111
P value: 0.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: PPFIA1
Fragment: 237-246
Site: 14-3-3_pSer242
Modification: (SEP)242
Sequence: RSSDGSLSHE
Construct: RSSDG(pSer)LSHE (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.09
Holdup BI standard deviation: 1.38
Immobilized partner concentration (10-6M): 111
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: PPFIA1
Fragment: 525-534
Site: 14-3-3_pSer530
Modification: (SEP)530
Sequence: RPHLGSVPDF
Construct: RPHLG(pSer)VPDF (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.24
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 111
P value: 2.88
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.45
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: PPFIA1
Fragment: 663-672
Site: 14-3-3_pSer668
Modification: (SEP)668
Sequence: RVGSGSLDNL
Construct: RVGSG(pSer)LDNL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.11
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 111
P value: 4.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: PPFIA1
Fragment: 758-767
Site: 14-3-3_pSer763
Modification: (SEP)763
Sequence: ALHTVSHEDI
Construct: ALHTV(pSer)HEDI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.21
Holdup BI standard deviation: 0.44
Immobilized partner concentration (10-6M): 111
P value: 0.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: PPFIA1
Fragment: 801-810
Site: 14-3-3_pSer806
Modification: (SEP)806
Sequence: KGIKSSIGRL
Construct: KGIKS(pSer)IGRL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.07
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 111
P value: 0.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: PPFIA1
Fragment: 1154-1163
Site: 14-3-3_pThr1159
Modification: (TPO)1159
Sequence: AGSAETLPAN
Construct: AGSAE(pThr)LPAN (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.35
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 111
P value: 3.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 22

Protein A

Protein: PPFIA1
Fragment: 567-576
Site: 14-3-3_pThr572
Modification: (TPO)572
Sequence: PSKVQTLNEQ
Construct: PSKVQ(pThr)LNEQ (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.08
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 111
P value: 0.91
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))