Interaction Details


Details of experiments between Q13045 and NC_000001.11
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FLII
Fragment: 1-1269
Construct: endogenous protein (K562)

Protein B

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC
Average holdup BI: 0.79
Immobilized partner concentration (10-6M): 5
P value: 8.1
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.87
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: FLII
Fragment: 1-1269
Construct: endogenous protein (K562)

Protein B

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC
Average holdup BI: 0.5
Immobilized partner concentration (10-6M): 5
P value: 5.67
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.31
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: FLII
Fragment: 1-1269
Construct: endogenous protein (K562)

Protein B

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC
Average holdup BI: 0.5
Immobilized partner concentration (10-6M): 5
P value: 5.73
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
Measured pKd: 5.3
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: FLII
Fragment: 1-1269
Construct: endogenous protein (K562)

Protein B

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 5
P value: 3.92
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))