Interaction Details


Details of experiments between Q13009 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TIAM1
Fragment: 835-935
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0.03
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835