Interaction Details


Details of experiments between Q13009 and Q9NZN5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TIAM1
Fragment: 1582-1591
Site: PBM
Sequence: APSRKLNTEI
Construct: Biotin-Ahx-APSRKLNTEI

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.76
PUBMED ID: 36115835