Interaction Details


Details of experiments between Q12959 and Q9UHC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL
Average holdup BI: 0.39
Normalized holdup BI: 0.58
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL
Average holdup BI: 0.44
Normalized holdup BI: 0.53
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.56
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.52
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL
Average holdup BI: 0.24
Normalized holdup BI: 0.27
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.34
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL
Average holdup BI: 0.19
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL
Average holdup BI: 0.26
Normalized holdup BI: 0.3
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.12
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 7

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL
Average holdup BI: 0.12
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 8

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL
Average holdup BI: 0.2
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.97
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 9

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.