Interaction Details


Details of experiments between Q12959 and Q8WXI2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CNKSR2
Fragment: 1025-1034
Site: PBM
Sequence: HTHSYIETHV
Construct: Biotin-Ahx-HTHSYIETHV
Average holdup BI: 0.55
Normalized holdup BI: 0.66
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.1
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CNKSR2
Fragment: 1025-1034
Site: PBM
Sequence: HTHSYIETHV
Construct: Biotin-Ahx-HTHSYIETHV
Average holdup BI: 0.31
Normalized holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.62
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CNKSR2
Fragment: 1025-1034
Site: PBM
Sequence: HTHSYIETHV
Construct: Biotin-Ahx-HTHSYIETHV
Average holdup BI: 0.09
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.85
PUBMED ID: 36115835