Interaction Details


Details of experiments between Q12959 and Q15311
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.55
Normalized holdup BI: 0.61
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.01
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 1-655
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.45
Immobilized partner concentration (10-6M): 10
P value: 3.63
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.91
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.5
Normalized holdup BI: 0.56
Immobilized partner concentration (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.7
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.24
Normalized holdup BI: 0.36
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.54
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.28
Normalized holdup BI: 0.34
Immobilized partner concentration (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.28
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.22
Immobilized partner concentration (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.17
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.17
Normalized holdup BI: 0.19
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.15
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 9

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.12
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.79
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 1-655
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 10
P value: 0.72
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 11

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835