Interaction Details


Details of experiments between Q12959 and Q13224
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIN2B
Fragment: 1475-1484
Site: PBM
Sequence: EKLSSIESDV
Construct: Biotin-Ahx-EKLSSIESDV
Average holdup BI: 0.5
Normalized holdup BI: 0.59
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.97
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIN2B
Fragment: 1475-1484
Site: PBM
Sequence: EKLSSIESDV
Construct: Biotin-Ahx-EKLSSIESDV
Average holdup BI: 0.2
Normalized holdup BI: 0.25
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.3
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIN2B
Fragment: 1475-1484
Site: PBM
Sequence: EKLSSIESDV
Construct: Biotin-Ahx-EKLSSIESDV
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835