Interaction Details


Details of experiments between Q12959 and P78310
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CXADR
Fragment: 1-365
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.76
Immobilized partner concentration (10-6M): 10
P value: 2.32
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.5
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: CXADR
Fragment: 1-365
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.75
Immobilized partner concentration (10-6M): 10
P value: 2.26
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 5.47
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))