Interaction Details


Details of experiments between Q12959 and P56282
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 1-904
Construct: endogenous proteins (Jurkat)

Protein B

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein
Average holdup BI: -0.11
Immobilized partner concentration (10-6M): 26
P value: 1.74
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18

Experiment series 2

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: POLE2
Fragment: 1-527
Construct: endogenous protein (Jurkat)
Average holdup BI: -0.25
Immobilized partner concentration (10-6M): 10
P value: 0.51
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: POLE2
Fragment: 1-527
Construct: endogenous protein (Jurkat)
Average holdup BI: -0.35
Immobilized partner concentration (10-6M): 10
P value: 0.73
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))