Interaction Details


Details of experiments between Q12959 and P41252
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: IARS1
Fragment: 1-1262
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.19
Immobilized partner concentration (10-6M): 10
P value: 7.77
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
Measured pKd: 4.38
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Protein B

Protein: IARS1
Fragment: 1-1262
Construct: endogenous protein (Jurkat)
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
P value: 0.38
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))