Interaction Details


Details of experiments between Q12959 and P36809
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.72
Normalized holdup BI: 0.81
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.63
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.47
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.63
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.41
Normalized holdup BI: 0.49
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.93
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.84
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.2
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.39
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835