Interaction Details


Details of experiments between Q12959 and P27037
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.32
Normalized holdup BI: 0.36
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.24
Normalized holdup BI: 0.29
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.11
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.12
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.71
PUBMED ID: 36115835