Interaction Details


Details of experiments between Q12959 and O95477
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.63
Normalized holdup BI: 0.7
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.19
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.55
Normalized holdup BI: 0.66
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.09
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.35
Normalized holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.62
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.3
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.6
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.07
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.84
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.07
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.76
PUBMED ID: 36115835