Interaction Details


Details of experiments between Q12923 and A6PVI3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PTPN13
Fragment: 1085-1188
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NCBP2L
Fragment: 144-153
Site: PBM
Sequence: RGGFGRQTQI
Construct: biotin-ado-ado-RGGFGRQTQI
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.71
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.