Interaction Details


Details of experiments between Q09666 and Q96BN8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AHNAK1
Fragment: 2-99
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: biotin-ttds-PVRVCEETSL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835