Interaction Details


Details of experiments between Q09666 and Q6ZMN7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AHNAK1
Fragment: 2-99
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDZRN4
Fragment: 1027-1036
Site: PBM
Sequence: HNAFLSVTTV
Construct: Biotin-Ahx-HNAFLSVTTV
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.