Interaction Details


Details of experiments between Q09666 and Q68D42
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AHNAK1
Fragment: 2-99
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TMEM215
Fragment: 226-235
Site: PBM
Sequence: QGRWDHETIV
Construct: Biotin-Ahx-QGRWDHETIV
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.64
PUBMED ID: 36115835