Interaction Details


Details of experiments between Q07666 and P00519
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KHDRBS1
Fragment: 1-443
Construct: endogenous protein (Jurkat)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.3
Immobilized partner concentration (10-6M): 10
P value: 6.1
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.63
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: KHDRBS1
Fragment: 421-435
Site: PxxP_3
Sequence: PSLKAPPARPVKGAY
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 3.49
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: KHDRBS1
Fragment: 421-435
Site: PxxP_3
Sequence: PSLKAPPARPVKGAY
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 3.49
PUBMED ID: N

Experiment series 4

Protein A

Protein: KHDRBS1
Fragment: 32-46
Site: PxxP_1
Sequence: QTPSRQPPLPHRSRG
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.48
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: KHDRBS1
Fragment: 32-46
Site: PxxP_1
Sequence: QTPSRQPPLPHRSRG
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.48
PUBMED ID: N

Experiment series 6

Protein A

Protein: KHDRBS1
Fragment: 328-342
Site: PxxP_2
Sequence: TVTRGVPPPPTVRGA
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: KHDRBS1
Fragment: 328-342
Site: PxxP_2
Sequence: TVTRGVPPPPTVRGA
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N