Interaction Details


Details of experiments between Q07157 and Q9IR59
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TJP1
Fragment: 14-113
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TJP1
Fragment: 184-264
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.11
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: TJP1
Fragment: 184-264
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.02
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: TJP1
Fragment: 415-516
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.07
Normalized holdup BI: -0.07
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: TJP1
Fragment: 415-516
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835