Interaction Details

Details of experiments between Q07157 and Q14155
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: TJP1
Fragment: 184-264
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF7
Fragment: 794-803
Site: PBM
Sequence: NDPAWDETNL
Construct: biotin-ttds-NDPAWDETNL

Average holdup BI: 0.02

Holdup BI standard deviation: 0

Normalized holdup BI: 0.02

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 2