Interaction Details


Details of experiments between Q07002 and P63104
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CDK18
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: MSRRASLSDI
Construct: MSRRA(pSer)LSDI (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.49
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 132
P value: 5.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.86
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: CDK18
Fragment: 11-20
Site: 14-3-3_pSer16
Modification: (SEP)16
Sequence: RRFSLSVPRT
Construct: RRFSL(pSer)VPRT (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.82
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 132
P value: 4.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.54
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: CDK18
Fragment: 84-93
Site: 14-3-3_pSer89
Modification: (SEP)89
Sequence: NQRRFSMEDV
Construct: NQRRF(pSer)MEDV (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.14
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 132
P value: 2.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: CDK18
Fragment: 93-102
Site: 14-3-3_pSer98
Modification: (SEP)98
Sequence: VSKRLSLPMD
Construct: VSKRL(pSer)LPMD (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.39
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 132
P value: 4.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: CDK18
Fragment: 152-161
Site: 14-3-3_pThr157
Modification: (TPO)157
Sequence: EGTYATVFKG
Construct: EGTYA(pThr)VFKG (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.56
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 132
P value: 7.43
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.97
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))