Interaction Details


Details of experiments between Q07002 and P27348
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CDK18
Fragment: 127-136
Site: 14-3-3_pSer132
Modification: (SEP)132
Sequence: MSRRASLSDI
Construct: MSRRA(pSer)LSDI (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.35
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 4.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.61
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: CDK18
Fragment: 11-20
Site: 14-3-3_pSer16
Modification: (SEP)16
Sequence: RRFSLSVPRT
Construct: RRFSL(pSer)VPRT (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.76
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 4.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: CDK18
Fragment: 84-93
Site: 14-3-3_pSer89
Modification: (SEP)89
Sequence: NQRRFSMEDV
Construct: NQRRF(pSer)MEDV (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.12
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 130
P value: 1.47
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: CDK18
Fragment: 93-102
Site: 14-3-3_pSer98
Modification: (SEP)98
Sequence: VSKRLSLPMD
Construct: VSKRL(pSer)LPMD (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.37
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 130
P value: 3.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.66
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: CDK18
Fragment: 152-161
Site: 14-3-3_pThr157
Modification: (TPO)157
Sequence: EGTYATVFKG
Construct: EGTYA(pThr)VFKG (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.6
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 7.85
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.06
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))