Interaction Details


Details of experiments between Q05586 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRIN1
Fragment: 929-938
Site: PBM
Sequence: LSDPSVSTVV
Construct: Biotin-Ahx-LSDPSVSTVV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.23
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.25
PUBMED ID: 36115835