Interaction Details


Details of experiments between Q04917 and Q6WCQ1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: MPRIP
Fragment: 1011-1020
Site: 14-3-3_pSer1016
Modification: (SEP)1016
Sequence: NIRSKSVIEQ
Construct: NIRSK(pSer)VIEQ (crude synthetic peptide)
Average holdup BI: 0.55
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 6.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.97
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: MPRIP
Fragment: 264-273
Site: 14-3-3_pSer269
Modification: (SEP)269
Sequence: ESGYFSLEKT
Construct: ESGYF(pSer)LEKT (crude synthetic peptide)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 3.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: MPRIP
Fragment: 289-298
Site: 14-3-3_pSer294
Modification: (SEP)294
Sequence: SPPSPSTPNH
Construct: SPPSP(pSer)TPNH (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 3.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: MPRIP
Fragment: 988-997
Site: 14-3-3_pSer993
Modification: (SEP)993
Sequence: IMKSKSNPDF
Construct: IMKSK(pSer)NPDF (crude synthetic peptide)
Average holdup BI: 0.99
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 130
P value: 7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 6.15
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: MPRIP
Fragment: 373-382
Site: 14-3-3_pThr378
Modification: (TPO)378
Sequence: LDRRSTEPSV
Construct: LDRRS(pThr)EPSV (crude synthetic peptide)
Average holdup BI: 0.66
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 7.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.17
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: MPRIP
Fragment: 537-546
Site: 14-3-3_pThr542
Modification: (TPO)542
Sequence: EGRSKTFDWA
Construct: EGRSK(pThr)FDWA (crude synthetic peptide)
Average holdup BI: 0.68
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 6.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.21
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))