Interaction Details


Details of experiments between Q04917 and Q5JSL3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 7-16
Site: 14-3-3_pSer12
Modification: (SEP)12
Sequence: FTKRLSKPGT
Construct: FTKRL(pSer)KPGT (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 0.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 1232-1241
Site: 14-3-3_pSer1237
Modification: (SEP)1237
Sequence: IKREDSRGSL
Construct: IKRED(pSer)RGSL (crude synthetic peptide)
Average holdup BI: 0.35
Holdup BI standard deviation: 0.24
Immobilized partner concentration (10-6M): 130
P value: 1.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.63
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 324-333
Site: 14-3-3_pSer329
Modification: (SEP)329
Sequence: QLNKLSRGDG
Construct: QLNKL(pSer)RGDG (crude synthetic peptide)
Average holdup BI: -0.17
Holdup BI standard deviation: 0.26
Immobilized partner concentration (10-6M): 130
P value: 0.74
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 495-504
Site: 14-3-3_pSer500
Modification: (SEP)500
Sequence: PYIKNSDPVK
Construct: PYIKN(pSer)DPVK (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 130
P value: 0.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: KTQIYSDPLR
Construct: KTQIY(pSer)DPLR (crude synthetic peptide)
Average holdup BI: 0.87
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 6.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.72
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 1347-1356
Site: 14-3-3_pThr1352
Modification: (TPO)1352
Sequence: DRKSQTMPAL
Construct: DRKSQ(pThr)MPAL (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 6.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.46
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: DOCK11
Fragment: 82-91
Site: 14-3-3_pThr87
Modification: (TPO)87
Sequence: RTVQSTVPED
Construct: RTVQS(pThr)VPED (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))