Interaction Details


Details of experiments between Q04917 and Q13009
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: TIAM1
Fragment: 1402-1411
Site: 14-3-3_pSer1407
Modification: (SEP)1407
Sequence: LLKTESLPSS
Construct: LLKTE(pSer)LPSS (crude synthetic peptide)
Average holdup BI: 0.74
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 6.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.34
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: TIAM1
Fragment: 1432-1441
Site: 14-3-3_pSer1437
Modification: (SEP)1437
Sequence: MSRAVSAPSK
Construct: MSRAV(pSer)APSK (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 8.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.18
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: TIAM1
Fragment: 167-176
Site: 14-3-3_pSer172
Modification: (SEP)172
Sequence: KKRSKSADIW
Construct: KKRSK(pSer)ADIW (crude synthetic peptide)
Average holdup BI: -0.41
Holdup BI standard deviation: 1.15
Immobilized partner concentration (10-6M): 130
P value: 0.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: TIAM1
Fragment: 329-338
Site: 14-3-3_pSer334
Modification: (SEP)334
Sequence: SEFADSGIEG
Construct: SEFAD(pSer)GIEG (crude synthetic peptide)
Average holdup BI: -0.15
Holdup BI standard deviation: 0.5
Immobilized partner concentration (10-6M): 130
P value: 0.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: TIAM1
Fragment: 55-64
Site: 14-3-3_pSer60
Modification: (SEP)60
Sequence: STRSSSTPSI
Construct: STRSS(pSer)TPSI (crude synthetic peptide)
Average holdup BI: 0.79
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 130
P value: 7.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.45
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: TIAM1
Fragment: 314-323
Site: 14-3-3_pThr319
Modification: (TPO)319
Sequence: GRRAKTTQDV
Construct: GRRAK(pThr)TQDV (crude synthetic peptide)
Average holdup BI: 0.82
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 130
P value: 4.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.54
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))