Interaction Details


Details of experiments between Q04917 and P46937
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 144-153
Site: 14-3-3_pSer149
Modification: (SEP)149
Sequence: PTGVVSGPAA
Construct: PTGVV(pSer)GPAA (crude synthetic peptide)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 130
P value: 3.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.77
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 271-280
Site: 14-3-3_pSer276
Modification: (SEP)276
Sequence: QRISQSAPVK
Construct: QRISQ(pSer)APVK (crude synthetic peptide)
Average holdup BI: 0.99
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 130
P value: 11.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 6.02
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 271-280
Site: 14-3-3_pSer276
Modification: (SEP)276
Sequence: QRISQSAPVK
Construct: biotin-ttds-QRISQ(pSer)APVK (HPLC-purified synthetic peptide)
Measured dissociation constant (10-6M): 2.9
Standard deviation of dissociation constant (10-6M): 0.2
Experiment method: competitive Fluorescence Polarization
Details of affinity fitting: Competitive binding equation in ProFit
Date of measurement: 2022-01-19
Number of measurements: 3
Measured pKd: 5.53
DOI: 10.1101/2022.12.08.519103

Experiment series 4

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 366-375
Site: 14-3-3_pSer371
Modification: (SEP)371
Sequence: SSPGMSQELR
Construct: SSPGM(pSer)QELR (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 0.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 376-385
Site: 14-3-3_pSer381
Modification: (SEP)381
Sequence: TMTTNSSDPF
Construct: TMTTN(pSer)SDPF (crude synthetic peptide)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.14
Immobilized partner concentration (10-6M): 130
P value: 1.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 382-391
Site: 14-3-3_pSer387
Modification: (SEP)387
Sequence: TDSGLSMSSY
Construct: TDSGL(pSer)MSSY (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 130
P value: 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 383-392
Site: 14-3-3_pSer388
Modification: (SEP)388
Sequence: DPFLNSGTYH
Construct: DPFLN(pSer)GTYH (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 130
P value: 0.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 56-65
Site: 14-3-3_pSer61
Modification: (SEP)61
Sequence: HVRGDSETDL
Construct: HVRGD(pSer)ETDL (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.55
Immobilized partner concentration (10-6M): 130
P value: 0
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 140-149
Site: 14-3-3_pThr145
Modification: (TPO)145
Sequence: GTLTPTGVVS
Construct: GTLTP(pThr)GVVS (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 130
P value: 0.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 72-81
Site: 14-3-3_pThr77
Modification: (TPO)77
Sequence: VMNPKTANVP
Construct: VMNPK(pThr)ANVP (crude synthetic peptide)
Measured dissociation constant (10-6M): <1
Immobilized partner concentration (10-6M): 130
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 1
Measured pKd: 6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Protein B

Protein: YAP1
Fragment: 78-87
Site: 14-3-3_pThr83
Modification: (TPO)83
Sequence: ANVPQTVPMR
Construct: ANVPQ(pThr)VPMR (crude synthetic peptide)
Average holdup BI: 0.75
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 130
P value: 6.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.35
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))