Interaction Details


Details of experiments between Q02505 and Q9Y566
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MUC3A
Fragment: 3314-3323
Site: PBM
Sequence: KRPEMTSSSV
Construct: biotin-ado-ado-KRPEMTSSSV

Protein B

Protein: SHANK1
Fragment: 655-761
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.95
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.