Interaction Details


Details of experiments between Q02410 and Q68EM7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP17
Fragment: 872-881
Site: PBM
Sequence: IDNDTESTAL
Construct: biotin-ttds-IDNDTESTAL
Average holdup BI: 0.09
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.89
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP17
Fragment: 872-881
Site: PBM
Sequence: IDNDTESTAL
Construct: biotin-ttds-IDNDTESTAL
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835