Interaction Details


Details of experiments between Q02410 and P36762
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL
Average holdup BI: 0.12
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.71
PUBMED ID: 36115835