Interaction Details


Details of experiments between Q02410 and P25054
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV
Average holdup BI: 0.13
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: APBA1
Fragment: 743-822
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV
Average holdup BI: 0.14
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: APBA1
Fragment: 655-741
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: APC
Fragment: 2834-2843
Site: PBM
Sequence: HSGSYLVTSV
Construct: Biotin-Ahx-HSGSYLVTSV
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6