Interaction Details


Details of experiments between Q02241 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KIF23
Fragment: 155-164
Site: 14-3-3_pSer160
Modification: (SEP)160
Sequence: SNDRNSMDIQ
Construct: SNDRN(pSer)MDIQ (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.35
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 134
P value: 2.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: KIF23
Fragment: 155-164
Site: 14-3-3_pSer160
Modification: (SEP)160
Sequence: SNDRNSMDIQ
Construct: SNDRN(pSer)MDIQ (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.06
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 102
P value: 0.87
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: KIF23
Fragment: 13-22
Site: 14-3-3_pSer18
Modification: (SEP)18
Sequence: TVKKGSQTNL
Construct: TVKKG(pSer)QTNL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.04
Holdup BI standard deviation: 0.39
Immobilized partner concentration (10-6M): 102
P value: 0.08
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: KIF23
Fragment: 677-686
Site: 14-3-3_pSer682
Modification: (SEP)682
Sequence: KVTQRSVSPS
Construct: KVTQR(pSer)VSPS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.2
Holdup BI standard deviation: 0.46
Immobilized partner concentration (10-6M): 102
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: KIF23
Fragment: 938-947
Site: 14-3-3_pSer943
Modification: (SEP)943
Sequence: EMRAGSQLGP
Construct: EMRAG(pSer)QLGP (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.25
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 134
P value: 2.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: KIF23
Fragment: 938-947
Site: 14-3-3_pSer943
Modification: (SEP)943
Sequence: EMRAGSQLGP
Construct: EMRAG(pSer)QLGP (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.07
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 102
P value: 1.37
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: KIF23
Fragment: 892-901
Site: 14-3-3_pThr897
Modification: (TPO)897
Sequence: FTDIETLKQE
Construct: FTDIE(pThr)LKQE (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 0.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: KIF23
Fragment: 892-901
Site: 14-3-3_pThr897
Modification: (TPO)897
Sequence: FTDIETLKQE
Construct: FTDIE(pThr)LKQE (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.05
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 102
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))