Interaction Details


Details of experiments between Q00537 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CDK17
Fragment: 100-109
Site: 14-3-3_pSer105
Modification: (SEP)105
Sequence: NLKMGSDGES
Construct: NLKMG(pSer)DGES (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.22
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 134
P value: 1.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: CDK17
Fragment: 100-109
Site: 14-3-3_pSer105
Modification: (SEP)105
Sequence: NLKMGSDGES
Construct: NLKMG(pSer)DGES (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.1
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 102
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: CDK17
Fragment: 132-141
Site: 14-3-3_pSer137
Modification: (SEP)137
Sequence: IHRRISMEDL
Construct: IHRRI(pSer)MEDL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.03
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 102
P value: 0.33
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: CDK17
Fragment: 141-150
Site: 14-3-3_pSer146
Modification: (SEP)146
Sequence: LNKRLSLPAD
Construct: LNKRL(pSer)LPAD (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.49
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 102
P value: 6.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.98
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: CDK17
Fragment: 141-150
Site: 14-3-3_pSer146
Modification: (SEP)146
Sequence: LNKRLSLPAD
Construct: LNKRL(pSer)LPAD (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.52
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 5.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.91
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: CDK17
Fragment: 175-184
Site: 14-3-3_pSer180
Modification: (SEP)180
Sequence: RSRRASLSEI
Construct: RSRRA(pSer)LSEI (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.5
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 102
P value: 6.7
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.99
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: CDK17
Fragment: 175-184
Site: 14-3-3_pSer180
Modification: (SEP)180
Sequence: RSRRASLSEI
Construct: RSRRA(pSer)LSEI (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.11
Holdup BI standard deviation: 0.63
Immobilized partner concentration (10-6M): 134
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: CDK17
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: APRLDSEGIE
Construct: APRLD(pSer)EGIE (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.07
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 2.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: CDK17
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: APRLDSEGIE
Construct: APRLD(pSer)EGIE (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.06
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 102
P value: 0.87
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: CDK17
Fragment: 515-524
Site: 14-3-3_pSer520
Modification: (SEP)520
Sequence: KNRRQSMLF-
Construct: GKNRRQ(pSer)MLF (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.94
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 1.89
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.05
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: CDK17
Fragment: 515-524
Site: 14-3-3_pSer520
Modification: (SEP)520
Sequence: KNRRQSMLF-
Construct: GKNRRQ(pSer)MLF (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.65
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 102
P value: 4.48
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.26
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 12

Protein A

Protein: CDK17
Fragment: 70-79
Site: 14-3-3_pSer75
Modification: (SEP)75
Sequence: LRRPHSVIGG
Construct: LRRPH(pSer)VIGG (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.88
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 102
P value: 6.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.88
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 13

Protein A

Protein: CDK17
Fragment: 70-79
Site: 14-3-3_pSer75
Modification: (SEP)75
Sequence: LRRPHSVIGG
Construct: LRRPH(pSer)VIGG (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.9
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 6.66
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.82
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: CDK17
Fragment: 4-13
Site: 14-3-3_pSer9
Modification: (SEP)9
Sequence: FKRRLSLTLR
Construct: FKRRL(pSer)LTLR (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.02
Holdup BI standard deviation: 0.85
Immobilized partner concentration (10-6M): 134
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 15

Protein A

Protein: CDK17
Fragment: 4-13
Site: 14-3-3_pSer9
Modification: (SEP)9
Sequence: FKRRLSLTLR
Construct: FKRRL(pSer)LTLR (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.2
Holdup BI standard deviation: 0.81
Immobilized partner concentration (10-6M): 102
P value: 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 16

Protein A

Protein: CDK17
Fragment: 87-96
Site: 14-3-3_pSer92
Modification: (SEP)92
Sequence: MPRNGSRLDI
Construct: MPRNG(pSer)RLDI (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.59
Holdup BI standard deviation: 0.25
Immobilized partner concentration (10-6M): 134
P value: 1.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.03
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 17

Protein A

Protein: CDK17
Fragment: 87-96
Site: 14-3-3_pSer92
Modification: (SEP)92
Sequence: MPRNGSRLDI
Construct: MPRNG(pSer)RLDI (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.05
Holdup BI standard deviation: 0.34
Immobilized partner concentration (10-6M): 102
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 18

Protein A

Protein: CDK17
Fragment: 197-206
Site: 14-3-3_pThr202
Modification: (TPO)202
Sequence: KLGEGTYATV
Construct: KLGEG(pThr)YATV (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.14
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 3.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: CDK17
Fragment: 197-206
Site: 14-3-3_pThr202
Modification: (TPO)202
Sequence: KLGEGTYATV
Construct: KLGEG(pThr)YATV (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.19
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 102
P value: 2.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 20

Protein A

Protein: CDK17
Fragment: 200-209
Site: 14-3-3_pThr205
Modification: (TPO)205
Sequence: EGTYATVYKG
Construct: EGTYA(pThr)VYKG (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.66
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 102
P value: 6.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.27
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 21

Protein A

Protein: CDK17
Fragment: 200-209
Site: 14-3-3_pThr205
Modification: (TPO)205
Sequence: EGTYATVYKG
Construct: EGTYA(pThr)VYKG (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.67
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 7.52
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.19
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))