Interaction Details


Details of experiments between P98088 and P55345
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MUC5AC
Fragment: 1563-1577
Site: PxxP_2
Sequence: VSSKPTPTEPSTSSC
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.6
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: MUC5AC
Fragment: 1563-1577
Site: PxxP_2
Sequence: VSSKPTPTEPSTSSC
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.6
PUBMED ID: N

Experiment series 3

Protein A

Protein: MUC5AC
Fragment: 1311-1325
Site: PxxP_1
Sequence: IERRVYPCSPTTPVP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: MUC5AC
Fragment: 1311-1325
Site: PxxP_1
Sequence: IERRVYPCSPTTPVP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 5

Protein A

Protein: MUC5AC
Fragment: 1936-1950
Site: PxxP_3
Sequence: VSSKPTPTEPSTSSC
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: MUC5AC
Fragment: 1936-1950
Site: PxxP_3
Sequence: VSSKPTPTEPSTSSC
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 7

Protein A

Protein: MUC5AC
Fragment: 5643-5657
Site: PxxP_4
Sequence: SWERGVPVSPMH---
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: MUC5AC
Fragment: 5643-5657
Site: PxxP_4
Sequence: SWERGVPVSPMH---
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N