Interaction Details


Details of experiments between P83105 and Q9BSE5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HTRA4
Fragment: 368-476
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AGMAT
Fragment: 343-352
Site: PBM
Sequence: LCALPKVTTV
Construct: biotin-ttds-LCALPKVTTV
Average holdup BI: 0.43
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.43
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.66
PUBMED ID: 36115835