Interaction Details


Details of experiments between P78352 and Q6UVM3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.62
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Measured dissociation constant (10-6M): 240.56
Standard deviation of dissociation constant (10-6M): 64.17
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 3.62
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: -0.03
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0.04
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835