Interaction Details


Details of experiments between P78352 and P53778
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0.3
Normalized holdup BI: 0.38
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.25
Normalized holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.45
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0.17
Normalized holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.15
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0.16
Normalized holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.11
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.14
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.16
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.03
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835