Interaction Details


Details of experiments between P78352 and P36809
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.97
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.58
Normalized holdup BI: 0.74
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.45
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.41
Normalized holdup BI: 0.49
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.92
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.75
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.64
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Measured dissociation constant (10-6M): 35.51
Standard deviation of dissociation constant (10-6M): 1.36
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.45
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV30-E6
Fragment: 144-153
Site: PBM
Sequence: RHTTSTETAV
Construct: biotin-ttds-RHTTSTETAV
Average holdup BI: 0.19
Holdup BI standard deviation: 0
Normalized holdup BI: 0.22
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.35
PUBMED ID: 36115835