Interaction Details


Details of experiments between P78352 and A6NIM6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.14
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.29
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.11
Holdup BI standard deviation: 0
Normalized holdup BI: 0.15
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.22
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.18
Normalized holdup BI: 0.21
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.19
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.12
Normalized holdup BI: 0.14
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.19
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.12
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.13
Normalized holdup BI: 0.16
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.05
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 7

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M): 136.14
Standard deviation of dissociation constant (10-6M): 28.94
Average holdup BI: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 0
P value: 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 3.87
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 3.5
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 10

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 11

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 3.5
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 13

Protein A

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.03
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 11
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.1
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 3.5
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835