Interaction Details


Details of experiments between P63104 and Q9BZF3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: OSBPL6
Fragment: 285-294
Site: 14-3-3_pSer290
Modification: (SEP)290
Sequence: LQRTQSAPNF
Construct: LQRTQ(pSer)APNF (crude synthetic peptide)
Measured dissociation constant (10-6M): <1
Immobilized partner concentration (10-6M): 132
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 1
Measured pKd: 6
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: OSBPL6
Fragment: 39-48
Site: 14-3-3_pSer44
Modification: (SEP)44
Sequence: LERTASSSTE
Construct: LERTA(pSer)SSTE (crude synthetic peptide)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 132
P value: 0.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))