Interaction Details


Details of experiments between P63104 and Q6P1L5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: FAM117B
Fragment: 204-213
Site: 14-3-3_pSer209
Modification: (SEP)209
Sequence: TRQPSSSPSS
Construct: TRQPS(pSer)SPSS (crude synthetic peptide)
Average holdup BI: 0.45
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 132
P value: 1.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.8
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: FAM117B
Fragment: 215-224
Site: 14-3-3_pSer220
Modification: (SEP)220
Sequence: IRRTSSLDTL
Construct: IRRTS(pSer)LDTL (crude synthetic peptide)
Average holdup BI: 0.71
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 132
P value: 7.99
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.26
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: FAM117B
Fragment: 386-395
Site: 14-3-3_pSer391
Modification: (SEP)391
Sequence: SSSTRSIDTQ
Construct: SSSTR(pSer)IDTQ (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.28
Immobilized partner concentration (10-6M): 132
P value: 0.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: FAM117B
Fragment: 33-42
Site: 14-3-3_pThr38
Modification: (TPO)38
Sequence: QPMRATVPFQ
Construct: QPMRA(pThr)VPFQ (crude synthetic peptide)
Average holdup BI: 0.81
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 132
P value: 6.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.5
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: FAM117B
Fragment: 451-460
Site: 14-3-3_pThr456
Modification: (TPO)456
Sequence: LPKYATSPKP
Construct: LPKYA(pThr)SPKP (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 132
P value: 5.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.01
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))