Interaction Details


Details of experiments between P63104 and Q5VT97
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: SYDE2
Fragment: 1188-1197
Site: 14-3-3_pSer1193
Modification: (SEP)1193
Sequence: NKLNMSF---
Construct: LNKNKLNM(pSer)F (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 132
P value: 6.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.02
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: SYDE2
Fragment: 617-626
Site: 14-3-3_pSer622
Modification: (SEP)622
Sequence: KGGYLSDGDS
Construct: KGGYL(pSer)DGDS (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 132
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))