Interaction Details


Details of experiments between P63104 and Q13009
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: TIAM1
Fragment: 1402-1411
Site: 14-3-3_pSer1407
Modification: (SEP)1407
Sequence: LLKTESLPSS
Construct: LLKTE(pSer)LPSS (crude synthetic peptide)
Average holdup BI: 0.66
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 132
P value: 7.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.16
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: TIAM1
Fragment: 1432-1441
Site: 14-3-3_pSer1437
Modification: (SEP)1437
Sequence: MSRAVSAPSK
Construct: MSRAV(pSer)APSK (crude synthetic peptide)
Average holdup BI: 0.94
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 132
P value: 8.9
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.11
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: TIAM1
Fragment: 167-176
Site: 14-3-3_pSer172
Modification: (SEP)172
Sequence: KKRSKSADIW
Construct: KKRSK(pSer)ADIW (crude synthetic peptide)
Average holdup BI: -0.13
Holdup BI standard deviation: 1.01
Immobilized partner concentration (10-6M): 132
P value: 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: TIAM1
Fragment: 329-338
Site: 14-3-3_pSer334
Modification: (SEP)334
Sequence: SEFADSGIEG
Construct: SEFAD(pSer)GIEG (crude synthetic peptide)
Average holdup BI: -0.2
Holdup BI standard deviation: 0.54
Immobilized partner concentration (10-6M): 132
P value: 0.39
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: TIAM1
Fragment: 55-64
Site: 14-3-3_pSer60
Modification: (SEP)60
Sequence: STRSSSTPSI
Construct: STRSS(pSer)TPSI (crude synthetic peptide)
Average holdup BI: 0.75
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 132
P value: 11.96
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.35
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta

Protein B

Protein: TIAM1
Fragment: 314-323
Site: 14-3-3_pThr319
Modification: (TPO)319
Sequence: GRRAKTTQDV
Construct: GRRAK(pThr)TQDV (crude synthetic peptide)
Average holdup BI: 0.85
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 132
P value: 5.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.62
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))