Interaction Details


Details of experiments between P62258 and Q7Z309
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 126-135
Site: 14-3-3_pSer131
Modification: (SEP)131
Sequence: KMFVSSSGLP
Construct: KMFVS(pSer)SGLP (crude synthetic peptide)
Average holdup BI: -0.2
Holdup BI standard deviation: 1.47
Immobilized partner concentration (10-6M): 143
P value: 0.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 20-29
Site: 14-3-3_pSer25
Modification: (SEP)25
Sequence: LRRSSSAPLI
Construct: LRRSS(pSer)APLI (crude synthetic peptide)
Average holdup BI: 0.97
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 116
P value: 7.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.42
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 20-29
Site: 14-3-3_pSer25
Modification: (SEP)25
Sequence: LRRSSSAPLI
Construct: LRRSS(pSer)APLI (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 143
P value: 7.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.17
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 28-37
Site: 14-3-3_pSer33
Modification: (SEP)33
Sequence: LIHGLSDLSQ
Construct: LIHGL(pSer)DLSQ (crude synthetic peptide)
Average holdup BI: -0.08
Holdup BI standard deviation: 0.23
Immobilized partner concentration (10-6M): 116
P value: 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 28-37
Site: 14-3-3_pSer33
Modification: (SEP)33
Sequence: LIHGLSDLSQ
Construct: LIHGL(pSer)DLSQ (crude synthetic peptide)
Average holdup BI: -0.18
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 143
P value: 2.5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 45-54
Site: 14-3-3_pSer50
Modification: (SEP)50
Sequence: RTRRNSTTIM
Construct: RTRRN(pSer)TTIM (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.29
Immobilized partner concentration (10-6M): 143
P value: 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: IMSRHSLEEG
Construct: IMSRH(pSer)LEEG (crude synthetic peptide)
Average holdup BI: 0.19
Holdup BI standard deviation: 0.24
Immobilized partner concentration (10-6M): 116
P value: 0.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: IMSRHSLEEG
Construct: IMSRH(pSer)LEEG (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 143
P value: 0.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 116-125
Site: 14-3-3_pThr121
Modification: (TPO)121
Sequence: PAPSPTRGFG
Construct: PAPSP(pThr)RGFG (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 116
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM122B
Fragment: 116-125
Site: 14-3-3_pThr121
Modification: (TPO)121
Sequence: PAPSPTRGFG
Construct: PAPSP(pThr)RGFG (crude synthetic peptide)
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 143
P value: 0.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))