Interaction Details


Details of experiments between P62258 and Q6P1L5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 204-213
Site: 14-3-3_pSer209
Modification: (SEP)209
Sequence: TRQPSSSPSS
Construct: TRQPS(pSer)SPSS (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.63
Immobilized partner concentration (10-6M): 116
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 215-224
Site: 14-3-3_pSer220
Modification: (SEP)220
Sequence: IRRTSSLDTL
Construct: IRRTS(pSer)LDTL (crude synthetic peptide)
Average holdup BI: 0.52
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 143
P value: 7.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.87
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 215-224
Site: 14-3-3_pSer220
Modification: (SEP)220
Sequence: IRRTSSLDTL
Construct: IRRTS(pSer)LDTL (crude synthetic peptide)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 116
P value: 5.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.82
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 268-277
Site: 14-3-3_pSer273
Modification: (SEP)273
Sequence: HKRSASWGST
Construct: HKRSA(pSer)WGST (crude synthetic peptide)
Average holdup BI: -0.21
Holdup BI standard deviation: 0.94
Immobilized partner concentration (10-6M): 116
P value: 0.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 386-395
Site: 14-3-3_pSer391
Modification: (SEP)391
Sequence: SSSTRSIDTQ
Construct: SSSTR(pSer)IDTQ (crude synthetic peptide)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 116
P value: 0.54
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 386-395
Site: 14-3-3_pSer391
Modification: (SEP)391
Sequence: SSSTRSIDTQ
Construct: SSSTR(pSer)IDTQ (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.38
Immobilized partner concentration (10-6M): 143
P value: 0.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 33-42
Site: 14-3-3_pThr38
Modification: (TPO)38
Sequence: QPMRATVPFQ
Construct: QPMRA(pThr)VPFQ (crude synthetic peptide)
Average holdup BI: 0.76
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 116
P value: 4.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.44
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 33-42
Site: 14-3-3_pThr38
Modification: (TPO)38
Sequence: QPMRATVPFQ
Construct: QPMRA(pThr)VPFQ (crude synthetic peptide)
Average holdup BI: 0.76
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 143
P value: 5.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.34
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 451-460
Site: 14-3-3_pThr456
Modification: (TPO)456
Sequence: LPKYATSPKP
Construct: LPKYA(pThr)SPKP (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 143
P value: 5.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.95
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: FAM117B
Fragment: 451-460
Site: 14-3-3_pThr456
Modification: (TPO)456
Sequence: LPKYATSPKP
Construct: LPKYA(pThr)SPKP (crude synthetic peptide)
Average holdup BI: 0.88
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 116
P value: 8.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.8
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))