Interaction Details


Details of experiments between P62258 and Q5JSL3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 7-16
Site: 14-3-3_pSer12
Modification: (SEP)12
Sequence: FTKRLSKPGT
Construct: FTKRL(pSer)KPGT (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 116
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 7-16
Site: 14-3-3_pSer12
Modification: (SEP)12
Sequence: FTKRLSKPGT
Construct: FTKRL(pSer)KPGT (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 143
P value: 0.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 1232-1241
Site: 14-3-3_pSer1237
Modification: (SEP)1237
Sequence: IKREDSRGSL
Construct: IKRED(pSer)RGSL (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 116
P value: 0.77
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 1232-1241
Site: 14-3-3_pSer1237
Modification: (SEP)1237
Sequence: IKREDSRGSL
Construct: IKRED(pSer)RGSL (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.44
Immobilized partner concentration (10-6M): 143
P value: 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 324-333
Site: 14-3-3_pSer329
Modification: (SEP)329
Sequence: QLNKLSRGDG
Construct: QLNKL(pSer)RGDG (crude synthetic peptide)
Average holdup BI: -0.03
Holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 116
P value: 0.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 324-333
Site: 14-3-3_pSer329
Modification: (SEP)329
Sequence: QLNKLSRGDG
Construct: QLNKL(pSer)RGDG (crude synthetic peptide)
Average holdup BI: -0.16
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 143
P value: 0.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 495-504
Site: 14-3-3_pSer500
Modification: (SEP)500
Sequence: PYIKNSDPVK
Construct: PYIKN(pSer)DPVK (crude synthetic peptide)
Average holdup BI: 0.08
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 116
P value: 1.16
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 495-504
Site: 14-3-3_pSer500
Modification: (SEP)500
Sequence: PYIKNSDPVK
Construct: PYIKN(pSer)DPVK (crude synthetic peptide)
Average holdup BI: -0.12
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 143
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: KTQIYSDPLR
Construct: KTQIY(pSer)DPLR (crude synthetic peptide)
Average holdup BI: 0.85
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 116
P value: 8.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.7
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 10

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 53-62
Site: 14-3-3_pSer58
Modification: (SEP)58
Sequence: KTQIYSDPLR
Construct: KTQIY(pSer)DPLR (crude synthetic peptide)
Average holdup BI: 0.86
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 143
P value: 7.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.63
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 1347-1356
Site: 14-3-3_pThr1352
Modification: (TPO)1352
Sequence: DRKSQTMPAL
Construct: DRKSQ(pThr)MPAL (crude synthetic peptide)
Average holdup BI: 0.94
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 116
P value: 6.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.14
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 1347-1356
Site: 14-3-3_pThr1352
Modification: (TPO)1352
Sequence: DRKSQTMPAL
Construct: DRKSQ(pThr)MPAL (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 143
P value: 8.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.13
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 13

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 82-91
Site: 14-3-3_pThr87
Modification: (TPO)87
Sequence: RTVQSTVPED
Construct: RTVQS(pThr)VPED (crude synthetic peptide)
Average holdup BI: -0.01
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 116
P value: 0.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: DOCK11
Fragment: 82-91
Site: 14-3-3_pThr87
Modification: (TPO)87
Sequence: RTVQSTVPED
Construct: RTVQS(pThr)VPED (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 143
P value: 0.46
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))