Interaction Details


Details of experiments between P62258 and Q13094
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: LCP2
Fragment: 371-382
Site: 14-3-3_pSer376
Modification: (SEP)376
Sequence: FPQSASLPPY
Construct: FPQSA(pSer)LPPYFS (crude synthetic peptide)
Measured dissociation constant (10-6M): <10
Immobilized partner concentration (10-6M): 116
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 1
Measured pKd: 5
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: LCP2
Fragment: 371-382
Site: 14-3-3_pSer376
Modification: (SEP)376
Sequence: FPQSASLPPY
Construct: FPQSA(pSer)LPPYFS (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 143
P value: 2.92
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.98
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))