Interaction Details


Details of experiments between P62258 and Q13009
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 1402-1411
Site: 14-3-3_pSer1407
Modification: (SEP)1407
Sequence: LLKTESLPSS
Construct: LLKTE(pSer)LPSS (crude synthetic peptide)
Average holdup BI: 0.39
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 116
P value: 7.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.75
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 1402-1411
Site: 14-3-3_pSer1407
Modification: (SEP)1407
Sequence: LLKTESLPSS
Construct: LLKTE(pSer)LPSS (crude synthetic peptide)
Average holdup BI: 0.43
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 143
P value: 6.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.72
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 1432-1441
Site: 14-3-3_pSer1437
Modification: (SEP)1437
Sequence: MSRAVSAPSK
Construct: MSRAV(pSer)APSK (crude synthetic peptide)
Average holdup BI: 0.93
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 116
P value: 5.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.06
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 1432-1441
Site: 14-3-3_pSer1437
Modification: (SEP)1437
Sequence: MSRAVSAPSK
Construct: MSRAV(pSer)APSK (crude synthetic peptide)
Average holdup BI: 0.91
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 143
P value: 8.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.84
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 167-176
Site: 14-3-3_pSer172
Modification: (SEP)172
Sequence: KKRSKSADIW
Construct: KKRSK(pSer)ADIW (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.72
Immobilized partner concentration (10-6M): 116
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 329-338
Site: 14-3-3_pSer334
Modification: (SEP)334
Sequence: SEFADSGIEG
Construct: SEFAD(pSer)GIEG (crude synthetic peptide)
Average holdup BI: 0.2
Holdup BI standard deviation: 0.47
Immobilized partner concentration (10-6M): 116
P value: 0.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 55-64
Site: 14-3-3_pSer60
Modification: (SEP)60
Sequence: STRSSSTPSI
Construct: STRSS(pSer)TPSI (crude synthetic peptide)
Average holdup BI: 0.5
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 116
P value: 8.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.94
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 55-64
Site: 14-3-3_pSer60
Modification: (SEP)60
Sequence: STRSSSTPSI
Construct: STRSS(pSer)TPSI (crude synthetic peptide)
Average holdup BI: 0.53
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 143
P value: 9.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.9
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 9

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 690-699
Site: 14-3-3_pSer695
Modification: (SEP)695
Sequence: RSRFSSLWGL
Construct: RSRFS(pSer)LWGL (crude synthetic peptide)
Average holdup BI: 0.13
Holdup BI standard deviation: 0.74
Immobilized partner concentration (10-6M): 143
P value: 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 314-323
Site: 14-3-3_pThr319
Modification: (TPO)319
Sequence: GRRAKTTQDV
Construct: GRRAK(pThr)TQDV (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 143
P value: 5.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.39
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 11

Protein A

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon

Protein B

Protein: TIAM1
Fragment: 314-323
Site: 14-3-3_pThr319
Modification: (TPO)319
Sequence: GRRAKTTQDV
Construct: GRRAK(pThr)TQDV (crude synthetic peptide)
Average holdup BI: -0.15
Holdup BI standard deviation: 0.94
Immobilized partner concentration (10-6M): 116
P value: 0.12
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))