Interaction Details


Details of experiments between P61981 and Q00537
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 100-109
Site: 14-3-3_pSer105
Modification: (SEP)105
Sequence: NLKMGSDGES
Construct: NLKMG(pSer)DGES (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.27
Immobilized partner concentration (10-6M): 134
P value: 0.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 100-109
Site: 14-3-3_pSer105
Modification: (SEP)105
Sequence: NLKMGSDGES
Construct: NLKMG(pSer)DGES (crude synthetic peptide)
Average holdup BI: -0.11
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 129
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 100-109
Site: 14-3-3_pSer105
Modification: (SEP)105
Sequence: NLKMGSDGES
Construct: NLKMG(pSer)DGES (crude synthetic peptide)
Average holdup BI: -0.12
Holdup BI standard deviation: 0.5
Immobilized partner concentration (10-6M): 115
P value: 0.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 132-141
Site: 14-3-3_pSer137
Modification: (SEP)137
Sequence: IHRRISMEDL
Construct: IHRRI(pSer)MEDL (crude synthetic peptide)
Average holdup BI: -0.16
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 134
P value: 0.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 132-141
Site: 14-3-3_pSer137
Modification: (SEP)137
Sequence: IHRRISMEDL
Construct: IHRRI(pSer)MEDL (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 129
P value: 0.87
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 141-150
Site: 14-3-3_pSer146
Modification: (SEP)146
Sequence: LNKRLSLPAD
Construct: LNKRL(pSer)LPAD (crude synthetic peptide)
Average holdup BI: 0.61
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 129
P value: 6.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.09
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 141-150
Site: 14-3-3_pSer146
Modification: (SEP)146
Sequence: LNKRLSLPAD
Construct: LNKRL(pSer)LPAD (crude synthetic peptide)
Average holdup BI: 0.55
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 6.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.97
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 141-150
Site: 14-3-3_pSer146
Modification: (SEP)146
Sequence: LNKRLSLPAD
Construct: LNKRL(pSer)LPAD (crude synthetic peptide)
Average holdup BI: 0.64
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 7.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.2
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 175-184
Site: 14-3-3_pSer180
Modification: (SEP)180
Sequence: RSRRASLSEI
Construct: RSRRA(pSer)LSEI (crude synthetic peptide)
Average holdup BI: 0.72
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 129
P value: 5.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.29
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 10

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 175-184
Site: 14-3-3_pSer180
Modification: (SEP)180
Sequence: RSRRASLSEI
Construct: RSRRA(pSer)LSEI (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.48
Immobilized partner concentration (10-6M): 115
P value: 0.23
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 11

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: APRLDSEGIE
Construct: APRLD(pSer)EGIE (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 1.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 12

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: APRLDSEGIE
Construct: APRLD(pSer)EGIE (crude synthetic peptide)
Average holdup BI: -0.08
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 129
P value: 1.68
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 13

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: APRLDSEGIE
Construct: APRLD(pSer)EGIE (crude synthetic peptide)
Average holdup BI: -0.02
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 115
P value: 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 14

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 515-524
Site: 14-3-3_pSer520
Modification: (SEP)520
Sequence: KNRRQSMLF-
Construct: GKNRRQ(pSer)MLF (crude synthetic peptide)
Average holdup BI: 0.77
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 129
P value: 4.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.42
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 15

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 515-524
Site: 14-3-3_pSer520
Modification: (SEP)520
Sequence: KNRRQSMLF-
Construct: GKNRRQ(pSer)MLF (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration (10-6M): 134
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 1
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 16

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 70-79
Site: 14-3-3_pSer75
Modification: (SEP)75
Sequence: LRRPHSVIGG
Construct: LRRPH(pSer)VIGG (crude synthetic peptide)
Average holdup BI: 0.96
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 129
P value: 5.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 5.22
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 17

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 70-79
Site: 14-3-3_pSer75
Modification: (SEP)75
Sequence: LRRPHSVIGG
Construct: LRRPH(pSer)VIGG (crude synthetic peptide)
Average holdup BI: 0.95
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 7.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 5.11
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 18

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 70-79
Site: 14-3-3_pSer75
Modification: (SEP)75
Sequence: LRRPHSVIGG
Construct: LRRPH(pSer)VIGG (crude synthetic peptide)
Average holdup BI: 0.9
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 5.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.93
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 19

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 4-13
Site: 14-3-3_pSer9
Modification: (SEP)9
Sequence: FKRRLSLTLR
Construct: FKRRL(pSer)LTLR (crude synthetic peptide)
Average holdup BI: 0.22
Holdup BI standard deviation: 0.96
Immobilized partner concentration (10-6M): 134
P value: 0.16
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 20

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 4-13
Site: 14-3-3_pSer9
Modification: (SEP)9
Sequence: FKRRLSLTLR
Construct: FKRRL(pSer)LTLR (crude synthetic peptide)
Average holdup BI: 0.06
Holdup BI standard deviation: 0.99
Immobilized partner concentration (10-6M): 115
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 21

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 87-96
Site: 14-3-3_pSer92
Modification: (SEP)92
Sequence: MPRNGSRLDI
Construct: MPRNG(pSer)RLDI (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.47
Immobilized partner concentration (10-6M): 134
P value: 0.16
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 22

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 87-96
Site: 14-3-3_pSer92
Modification: (SEP)92
Sequence: MPRNGSRLDI
Construct: MPRNG(pSer)RLDI (crude synthetic peptide)
Average holdup BI: 0.23
Holdup BI standard deviation: 0.3
Immobilized partner concentration (10-6M): 129
P value: 0.69
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 23

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 197-206
Site: 14-3-3_pThr202
Modification: (TPO)202
Sequence: KLGEGTYATV
Construct: KLGEG(pThr)YATV (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 134
P value: 3.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 24

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 197-206
Site: 14-3-3_pThr202
Modification: (TPO)202
Sequence: KLGEGTYATV
Construct: KLGEG(pThr)YATV (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 129
P value: 4.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 25

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 197-206
Site: 14-3-3_pThr202
Modification: (TPO)202
Sequence: KLGEGTYATV
Construct: KLGEG(pThr)YATV (crude synthetic peptide)
Average holdup BI: 0.54
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 115
P value: 6.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.03
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 26

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 200-209
Site: 14-3-3_pThr205
Modification: (TPO)205
Sequence: EGTYATVYKG
Construct: EGTYA(pThr)VYKG (crude synthetic peptide)
Average holdup BI: 0.81
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 8.1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.51
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 27

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 200-209
Site: 14-3-3_pThr205
Modification: (TPO)205
Sequence: EGTYATVYKG
Construct: EGTYA(pThr)VYKG (crude synthetic peptide)
Average holdup BI: 0.78
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.44
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 28

Protein A

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma

Protein B

Protein: CDK17
Fragment: 200-209
Site: 14-3-3_pThr205
Modification: (TPO)205
Sequence: EGTYATVYKG
Construct: EGTYA(pThr)VYKG (crude synthetic peptide)
Average holdup BI: 0.7
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 7.77
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.33
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))